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表6底漆pairs for RT-qPCR and PCR ofCpMS1

From:Differential gene expression among three sex types reveals a MALE STERILITY 1 (CpMS1) for sex differentiation in papaya

Gene Primer F Primer R Tm (C)
Actin 2 TTTCCAAGGGTGAGTATGATGAG ACACAGGACACAAAAGCCAACTA 58
EIF 1 AGGCAGGCAAGAGAAGAT TTCATACCGAGTAGCGATTC 58
TBP 1 GGTAGTAGTAGTTAGGTATGTG GGCAATCTGGTCTCACTT 58
CpMS1 ATGGTCAGCTAAGCGAGTTG CGGGTTTAAGCTACGACGAA 58
CpMS1–1 TTGGAAATTAACAAAACGAGAAA TGGAGAGATTTCTTCAAAAGTTG 55
CpMS1–2 GCAATGGCCTCTTTGTTGTT TCCTGCCTTCCAAAAGATCA 55
CpMS1–3 TCAACTCGCTTAGCTGACCA TGTGAAGGTGGGTGTGATGT 55
CpMS14 AACCCACTTCGACTCCATTG TTTCTCCATTTGCAGTGTTTCTT 55