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Table 2 Candidate genes and their expression profiles as revealed by RNA-seq

From:Transcriptomic profiling of wheat near-isogenic lines reveals candidate genes on chromosome 3A for pre-harvest sprouting resistance

通用电气ne ID DEG at time point Physical location 通用电气ne annotation Pathway Expression detailsa
TraesCS3A01G461400 15, 25, 35 DPA 697,281,350
− 697,284,916
Fork head transcription factor 1 Purine metabolism Up regulated (log2 = 5.70)
TraesCS3A01G462000 15, 25, 35 DPA 697,471,602
−697,474,693
B3 domain-containing transcription factor Aminoacyl-tRNA biosynthesis Up regulated (log2 = 1.43)
TraesCS3A01G466700 15, 25, 35 DPA 700,562,650
−700,564,244
Hydroxyethylthiazole kinase Thiamine metabolism Down regulated (log2 = 5.19)
TraesCS3A01G459200 15, 25 DPA 695,744,627
- 695,745,649
Leucine-rich repeat receptor-like protein kinase family protein Fatty acid biosynthesis; starch and sucrose metabolism; phenylpropanoid biosynthesis; biosynthesis of secondary metabolites Up regulated (log2=2.14)
TraesCS3A01G245000 25, 35 DPA 458,679,119
− 458,682,490
Receptor kinase Plant hormone signal transduction Down regulated (log2 =4.00)
TraesCS3A01G225100 15 DPA 421,719,543
− 421,722,510
S-type anion channel MAPK signalling pathway - plant Up regulated (log2 =1.74)
  1. 通用电气nes in bold fell into the QTL (QPhs.ccsu-3A.1) marker interval of Xwmc153 and Xgwm155, with a physical position within 484,402,604–702,961,948 bp
  2. DPADays post-anthesis,Upregulated通用电气nes expressed higher in resistant isolines,Downregulated通用电气nes expressed higher in susceptible isolines,log2Mean log2 ratio fold-change of the DEG in both NIL pairs
  3. aThe upregulation and downregulation of DEGs is based on comparisons of resistant to susceptible isolines