| PCR marker |
Name |
Primer (5’ → 3’) |
Target allele(s) |
Expected pro-duct size (bp) |
Annealing temp. (°C) |
Reference. |
| VRN-A1markera |
Vrn1AF |
GAAAGGAAAAATTCTGCTCG |
VRN-A1f |
655 |
55 |
[13] |
| Int1R |
GCAGGAAATCGAAATCGAAG |
VRN-A1f-del |
| VRN-A1f-ins |
| VRN-A1f-del/ins |
| VRN-A1Non-deletion |
Intr1/C/F |
GCACTCCTAACCCACTAACC |
VRN-A1f |
1068 |
56 |
[14] |
| Intr1/AB/R |
TCATCCATCATCAAGGCAAA |
VRN-A1f-ins |
| VRN-A1insertion |
Intr 1 |
ATCATCTTCTCCACCAAGGG |
VRN-A1f |
1480 |
50 |
[15] |
| Intr1insR |
AATGAACAGCACGGAAACAG |
VRN-A1f-ins |
1910 |
|
|
| VRN-A1f-del/ins |
|
|
|
| mitef |
CAGGTAAGGTATGAGGTGAC |
VRN-A1f-ins |
370 |
52 |
- |
| miter |
GATTCCAAATGAGAAGATGAGG |
VRN-A1f-del/ins |
|
|
|
| VRN-A1deletion of 2.7 kb |
Intr1/A/F2 |
AGCCTCCACGGTTTGAAAGTAA |
VRN-A1f-del |
~2500 |
56 |
[14] |
| Intr4 |
GCGCCATTAGGGAGGCACTT |
VRN-A1f-del/ins |
|
|
|
| delf |
ACATGTAAGCAGATCCTATCGA |
VRN-A1f-del |
450 |
53 |
- |
| delr |
TGCTTTAGATCTTTCTTCACGG |
VRN-A1f-del/ins |
|
|
|
| VRN-G1markera |
| forT. araraticum/ |
P1 |
TACCCCTGCTACCAGTGCCT |
VRN-G1 |
900 |
55 |
[23] |
| T. timopheevii |
P5 |
GGCCAACCCTACACCCCAAG |
VRN-G1a |
1100 |
|
|
| forAe. speltoides |
P8 |
CTAGGACTGGCGAGTATCTT |
VRN-1 |
900 |
55 |
- |
| P10 |
GAGAACCGGGCCAACCCTAC |
|
|
|
|
| VRN-G1Non-deletion |
| forT. araraticum/ |
Ex1/C/F |
GTTCTCCACCGAGTCATGGT |
VRN-G1 |
1530 |
56 |
[14] |
| T. timopheevii |
Intr1/B/R4 |
CAAATGAAAAGGAATGAGAGCA |
VRN-G1a |
|
|
|
| forAe. speltoides |
Ex1/C/F |
GTTCTCCACCGAGTCATGGT |
VRN-1 |
1530 |
60 |
[14] |
| Intr1/D/R4 |
AAATGAAAAGGAACGAGAGCG |
|
|
|
|
- aThese diagnostic markers detect allelic variation at the promoter regions. In other cases variation within intron 1 of corresponding genes is detected
