Fig. 6

Morphological analysis of pollen and seed defects inp5cssesquimutants complemented byp₁₇₃₄₀:P5CS2expression.aPercentages of aberrant pollen grains andbpercentages of aberrant seeds in siliques from wildtype plants (Col-0),p5cssesquimutants (p5cs) and heterozygous or homozygous complementation lines (p₁₇₃₄₀:P5CS2het/hom). Bars represent the means ±SE of 16–22 samples per genotype inaand 9 siliques per genotype inbmixed from plants of two independent complementation lines. nd: not detected; Ina*** indicate significant differences fromp5cs, while inb*** indicate significant differences betweenp₁₇₃₄₀:P5CS2het andp₁₇₃₄₀:P5CS2hom (p < 0.001, by student’s T-test). Two further complementation lines produced similar results.c-fBright-field microscopic pictures of pollen grains from wildtypecandp5cssesquimutants withe-for withoutdthep₁₇₃₄₀:P5CSconstruct in homozygousfor heterozygous statee. An inset at higher magnification is also shown for thep5cssesquimutant and the heterozygous complementation line. Arrowheads indicate small and shriveled pollen grains.g-jStereomicroscopic images of opened, premature siliques from wildtypegandp5cssesquimutants withi-jor withouththep₁₇₃₄₀:P5CSconstruct. Aberrant seeds indicative of lethality ofp5cs2–1homozygous embryos are indicated by arrowheads