Fig. 1

Tissue-specific expression patterns ofBKN1andBKN2inA. thaliana.a.通用电气ne structures for the tandemly linkedBKN1andBKN2genes. The promoter regions used forBKNp:GUSconstructs are shown by blue arrows.b.RT-PCR analysis of different tissues showBKN1andBKN2expression in the stigmas. The double PCR bands observed forBKN1is the result of the third intron not being properly spliced in the top band (determined by sequencing). This is also seen in the carpel RNA-Seq mapping data (Fig.5).c-e.GUSstaining of different tissues fromBKN1p:GUStransgenic plants. GUS activity was specifically detected in stigmas across developmental stages in the inflorescence (c,d), and not in other tissues, including seedlings (e).f-h.GUS staining of different tissues fromBKN2p:GUStransgenic plants. GUS activity was detected primarily in the floral abscission zones and stems (f, g). GUS staining was also seen in the petioles and the tips of leaves for about half of the samples (h). Scale bars are 1 cm for inflorescence images (right), and 500 μm for stage 12 flowers (centre) and 100 μm for seedlings (left)