Fig. 3

Conserved residues distribution in the CLC gene family.aCLC gene tree with collapsed branches, and the conserved residues of each subfamily are listed on the right. Distinct residues are highlighted in red.bPartial sequence alignment of the seven CLC proteins in pomegranate. The conserved regions: GxGIPE (I), GKxGPxxH (II) and PxxGxLF (III) are circled in red color. Conserved E (Glu) residues are circled in blue color. The presence of the conserved gating glutamate (E) and/or proton glutamate (E) residues is a signature for distinguishing the CLC antiporters and CLC channels, respectively