图3.
使用QRT-PCR验证九种防御或样品特异性差异表达基因。对于RNA-SEQ，在5个生物学重复中测量每个样品中基因的标准化计数（归一化计数±SD）。对于QRT-PCR，每种样品中的相对基因表达（ΔCT±SD）在四个生物学重复中测量，并用泛素基因（OS03G0234200）用作参考基因的三种技术重复。OSICS1. = isochorismate synthase 1,Osaos2. = allene oxide synthase 2,奥斯拉克 = lipoxygenase,OSPAL1. = phenylalanine ammonia-lyase 1,OSPAL2. = phenylalanine ammonia-lyase 2,oschs1. = chalcone synthase 1, AK102138 = Bowman-Birk serine protease inhibitor family protein, expressed, AB246888 = NADPH oxidoreductase 1, AK064067 = conserved hypothetical protein, CL = control_leaf, CS = control_sheath, LL = leaffolder_leaf, LS = leaffolder_sheath, BL = BPH_leaf, BS=BPH_sheath. The measurements were analyzed with two-way ANOVA and LSD post hoc test with different letters denoting significant differences (P. < 0.05)