Figure 3

TheArabidopsisACR11- and ACR12-GFP fusion proteins are localized to the chloroplast.Arabidopsismesophyll protoplasts were transformed with ACR11- and ACR12-GFP constructs, which encode the full-length ACR11 protein, and the first 94 amino acids of ACR12 fused to GFP, respectively. Chloroplasts were visualized by red chlorophyll autofluorescence. The green fluorescent signals of ACR11- and ACR12-GFP colocalized with the red fluorescent signals of chlorophyll (merge).Arabidopsisprotoplasts transformed with the empty vector are shown as controls for the subcellular localization of GFP. Scale bars are 10 μm.