| Characteristic |
MdEXP1 |
MdEXP2 |
MdEXP3 |
MdEXP4 |
| Signal peptide |
1-24 |
1-23 |
1-19 |
1-24 |
| GH45 domain |
No |
39-180 |
52-211 |
No |
| Eight conserved cysteines |
Yes |
Yes |
Yes |
Yes |
| GGACGY motif |
GGsCGY |
GGsCGf |
GGsCGY |
GGsCGY |
| GxxCGxCF/Y motif |
Yes |
Yes |
Yes |
Yes |
| Y/FRRVPC motif |
IYAVSC |
FTQVPC |
VTRVPC |
VYAAGC |
| Catalytic site key residues |
No |
1 A |
1 A |
No |
| DPBB_1 domain |
51-132 |
56-136; 302-385 |
82-161 |
51-132 |
| Pollen_allerg_1 domain |
144-223 |
147-226 |
172-253 |
No |
| Four conserved tryptophan (W) residues (* structurally related residues) |
2(W) 1(F*) 1(Y*) |
2(W) 1(F*) 1(Y*) |
2(W) 2(Y*) |
No |
| HATFYG motif (A) |
No |
No |
No |
No |
| α-insertion (A) |
Yes |
Yes |
Yes |
Yes |
| β-insertion (B) |
No |
No |
No |
No |
| HFDL motif (A, B) |
Only L |
Only L |
Only L |
Only L |
| CDRC motif (LA) |
No |
No |
No |
No |
| Long carboxy terminal extension (LA) |
No |
Yes |
No |
No |
- When a domain is present, its position is given (starting from the first methionine). A, unique characteristic of the EXPA family; B, unique characteristic of the EXPB family; LA, unique characteristic of the EXLA family; LB, unique characteristic of the EXLB family
