| Substrate |
Product |
Vmax |
Km |
Vi/ Km |
|
|
(pmol.min-1.g-1FW) |
(μM) |
(μM.pmol-1.min-1.g-1FW) |
| p-coumaroyl quinate |
caffeoyl quinate |
0.49 +/- 0.16 |
7 +/- 5 |
0.07 +/- 0.02 |
| p-coumaroyl shikimate |
caffeoyl shikimate |
0.19 +/- 0.03 |
24 +/- 7 |
0.008 + / - 0.002 |
- Reactions were carried out at 28°C during 30 min in a KPi buffer 20Mm pH 7.0 as described in material and methods. Reactions were stopped with addition of 0.1% trifluoroacetic acid. Data obtained were fitted with the Michaelis-Menten equation.
