Figure 9

Transcript profiling, principle component analysis (PCA) and K-means clustering of 25 AAT genes.Distinct developmental phases were identified in flag leaves (A), glumes (B) and developing grains (C). Tissues were analysed in two-day steps starting 4 days before anthesis (-4) in flag leaves and glumes and at 4 DAF in developing grains until 24 DAF. The heat maps (upper panels) reflect relative transcript abundances after normalisation againstactinexpression (blue = low expression; red = high expression). Developmental phases as identified by PCA are given in the lower panels, numbers represent DAF. Results of K-means clustering are visualized by encircling of respective stages. Light violet areas represent the transition phase.