Figure 2
Expression of the mutatedA7一个dAS11 DGAT1一个d WTDGAT1cDNAs inH1246MATαyeast cells lacking DGAT1 activity. The insert photo (A) shows the western blot of the DGAT protein expressed in each lysate with the 52 kD and 75 kD Mrmarker positions indicated by the arrows. (B) DGAT activity in each lysate (measured as pmol14C triolein formed · min-1· (mg protein)-1in the presence of14C-1,2 diolein and 18:1-CoA) is reported as the average of triplicate determinations ± S.D. Only the expressed WT protein shows DGAT activity, confirming that both theAS11一个dA7DGAT1s are non-functional.