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Table 3 List of genes and isoenzymes used

From:The molecular genetic linkage map of the model legumeMedicago truncatula: an essential tool for comparative legume genomics and the isolation of agronomically important genes

Markers Nature Function Primer sequences (5'-3') RE LG References
ENBP1 SSR Chloroplastic protein L:CACTTCCCACTGTCCTAGTCCTACR:GACTCGTCATCACCAGTTTCATCC 1 [65]*
ENOD8 CAPS Nodulin L:ATGGTGCAAACTTCGCATCAGGAGGR:ACAACCCTTTGGTCCTGTGCCGTG SspI 1 [66]
MtPT-1 CAPS Phosphate transporter L:TTGCTAAGAACCCGAAACAAGCTGCR:ATGTTCATGATCCTTCTTGCTCAAGC RsaI 1 [13]
PEPC CAPS PhosphoEnolPyruvate Carboxylase L:CTCATCCTACTCAGTCGGTTCGTCGR:ACACGTAGCTCATCGTTGCAACGCC RsaI 2 [67]
Z-F1 RFLP Zn-Finger1 EcoRI 3 [68]
GS.B PCR Glutamine synthetase L:AGAAGTAGCGTGGTGGCR:GCTGCCTATGGAGAAGG 3 [69]
Mtlec2 SSR Lectine 2 L:AAAGAATTCCATCTTCCAAGGCGAR:GAAGTCTAGAAACCAATCCTTACCC 3 [70]
ENOD12 PCR Nodulin L:CCTGCTTATAGGCCACCACR:CTTCTGCTGGAGGATGCC 3 [71]
ATPase SSR Vacuolar ATPase L:GGGTTTTTGATCCAGATCTTR:AAGGTGGTCATACGAGCTCC 4 [15]**
MtST-1 PCR Sucrose transporter L:ATCAAAGGTGGATGGGGATGGAGR:TAGACCAGAAGGGATGTGATTTCC 4 [72]
PGM Isoenz. PhosphoGlucoMutase 4 [34]
Gs.C CAPS Glutamine synthetase L:GCACAAGGAGCACATTGR:GCCCATAATTAAACATCATG VspI 5 [69]
LbI CAPS Leghemoglobin I L:GATAGTCCTCAACTCCAAGCR:CCTGTTGCTCGAAGTTGAGA SspI 5 [73]
rDNA CAPS 45s Ribosomal DNA L:ATGGTCCGGTGAAGTGTTCGR:CCCGGTTCGCTCGCCGTTAC XbaI 5 这篇论文
ENOD40 CAPS Nodulin L:TTAGTAGGATCTTCTCTTTCACTAGCR:GCCTCCGATTATCAAAGGTCAAG AluI 5 [74]
GS.A PCR Glutamine synthetase L:AAAGACAAGTGGTTGTGACR:GCTGCCTATGGAGAAGG 6 [69]
MtN1 PCR Nodulin L:ATCTATCTTCTTCGCTCR:TGACTGTTGAACATCTC 6 [75]
GSHS SSR Glutathione synthetase L:AGCAATAGGCAATGGCTGCTCCTGCR:AATGGTGAGATGCTTATGATGAGAC 7 [76]
VR CAPS Vestitone reductase L:GAGTGTGTGTAACTGGAGGTACAGGR:ATGCCTAATGCGCCATCGACGGTTC DdeI 7 [77]
PGD Isoenz. PhosphoGlucoDeshydrogenase 7 [34]
ENOD16 RFLP Nodulin L:ATCGGGACACTCTTTAGCAAGACCCR:TGGGCGGTGGAATTGGACCTCTTG EcoRV 8 [78]
  1. Underlined markers have been used for synteny studies RE : Restriction Enzyme LG :M. truncatulaLinkage Group SSR : Simple Sequence Repeat: polymorphism is revealed by the length of the amplified fragment CAPS : Cleaved Amplified Polymorphic Sequence : polymorphism is revealed after digestion of the amplified fragment with restriction enzymes PCR : Polymerase Chain Reaction : polymorphism is revealed by the length of the amplified fragmentL: Left primerR: Right primer*: Accession n° AJ002479**: Accession n° AA660456
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BMC Plant Biology

ISSN: 1471-2229

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