Grandparents |
F2 family endogene silencing phenotype |
|
Endogene |
[%] cop |
Remarks1) |
|
cop |
wt |
Observed |
Expected |
Excess of |
L91' × E82 |
23 |
138 |
14% |
75% |
wt plants |
E82' × L91 |
30 |
42 |
42% |
27% |
cop plants |
L91 × E82 |
38 |
73 |
38% |
87% |
wt plants |
L91' × E82' |
1 |
17 |
6% |
0% |
one cop plant |
- 1)Data were Chi-square tested under the null-hypothesis that L91' and E82' do not trans-silence their target loci, i.e., an additive interaction between the partner loci. Thus, in the case of L91 × E82, the only genotype escaping cosuppression should be L91 /-; E82 (-/-), which occurs at a frequency of 2/15 (13%). Note that entirely non-transgenic plants are not considered because of counterselection. Imprinted loci are not expected to cause endogene silencing (see Fig.5). Thus, 12/15 of L91'/E82 family members, 4/15 of E82'/L91 family members and none of L91'/E82' family members are expected to be cop1-like. An excess of wild-type plants is inconsistent with an additive interaction and thus suggests trans-silencing activity by the L91' or L91 locus.