Figure 3
Introduction ofgusandnptII gene inT. aestivumcv. CPAN1676.A. PCR screening of genomic DNA samples of putative transformants ofTriticum aestivumcv CPAN1676 using primers specific togusgene. The transformants were obtained after bombardment of mature embryo-derived calli with pBI101::Act1. The plasmid pBI101::Act1 and the genomic DNA isolated from untransformed plant were used as the positive and the negative controls, respectively.B. Autoradiograph showing NPTII activity in dot blots of some of the T0transformants, C. Result of paromomycin leaf spray on T0transformants showing the functional activity ofnptII gene (Extreme left: control)