Figure 4

Southern blot hybridization analyses ofDsinsertion in thebfl1mutant andDsexcision in revertants.通用电气nomic DNA (~10 μg) was extracted from putative revertants, digested withHindIII, fractionated on a 0.7% agarose gel, and blotted to a Hybond-N⁺membrane. The membrane was hybridised with agusprobe (see Figure6). PR1 to PR10 are revertants of the originalbfl1mutant and PR2-1 is one of the progenies of PR2. Abbreviations: N, wild-type phenotype; M, mutant phenotype.