Figure 6

Genomic location ofBFL1andDsinsertion inbfl1mutant．Schematic representation of the positions of theBFL1gene and theDsinsertion inbfl1mutant in the Rice genomic PAC clone P0625E02 (GenBank Acc. No.AP004570). The orientation of theBFL1gene (P0625E02.25) is indicated by arrow. Binding sites of primers Ds5-112^-(P1), Ds3-6587⁺(P2), LW1125_Rev (P3), LW1125_For (P4) used in PCR1, PCR2 and PCR3, and OsBfl1_Rev2 (P5) and OsBfl1_For (P6) used in RT-PCR (see Table2for details) are indicated. Solid line representsgusprobe used for Southern blot hybridization analysis.HindIII restriction sites are indicated (H).