Figure 4
Synthesis ofL-[14C]galactono-1,4-lactone fromL-[1-14C]galactose in courgette fruit exudate.Radiochromatograms obtained from exudate incubated withL-[1-14C]galactose for 30 min at 30°C. The reaction was stopped by the addition of H3PO3prior to removal of precipitate and analysis by HPLC with online flow scintillation detection. Individual chromatograms show reactions containingL-[1-14C]galactose and heated (100 °C) exudate (A), fresh exudate (B), desalted exudate (C) and desalted exudate with addition of 0.5 mM NAD (D). The elution times of authenticL-[1-14C]galactose (L-Gal),L-[1-14C]galactonolactone (L-GalL) andL-[1-14C]galactonic acid (L-GalA) are shown.