| Activitya |
Phloem |
Tissue |
| HK (D-Glc) |
23.0 ± 1.73 |
22.5 ± 2.89 |
| HK (D-Frc) |
29.7 ± 1.33 |
24.8 ± 3.58 |
| HK (D-Man) |
4.0 ± 0.40 |
4.0 ± 0.58 |
| PGI |
1861.3 ± 15.18 |
1194.7 ± 173.38 |
| PMI |
94.2 ± 5.20 |
N.D.b |
| PMM |
1.5 ± 0.11 |
N.D.b |
| GDPM PPase |
22.8 ± 1.56 |
N.D.b |
| GDPM-3,5-epimerase |
0.07 ± 0.012 |
0.12 ± 0.017 |
| GDP-L-gal PPPase |
0.012 + 0.002 |
0.010 ± 0.002 |
| L-GalDH |
28.0 ± 0.46 |
7.0 ± 1.27 |
| L-GalLDH |
N.D.b |
1.98 ± 0.18 |
- aActivity is expressed in nmol min-1gFW-1of tissue ± S.E., n = 3bN.D. = Activity not detected Courgette fruit exudate or tissue was prepared and desalted as described in the text. Enzymes listed are: HK, hexokinase (E.C. 2.7.1.1) withD葡萄糖(D-Glc),D-fructose (D-Frc) orD-mannose (D-Man) as substrate; PGI, phosphoglucose isomerase (E.C. 5.3.1.9); PMI, phosphomannose isomerase (E.C. 5.3.1.8); PMM, phosphomannose mutase (E.C. 5.4.2.8); GDPM PPase, GDP-D-mannose pyrophosphorylase (E.C. 2.7.7.22); GDPM-3,5-epimerase, GDP-D-mannose-3,5-epimerase (E.C. 5.1.3.18); GDP-L-gal PPPase, GDP-L-galactose pyrophosphatase;L-GalDH,L半乳糖dehydrogenase;L-GalLDH,L-galactono-1,4-lactone dehydrogenase (E.C. 1.3.2.3). Where no E.C. numbers are supplied they have yet to be assigned.
