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Table 3 Conversion of14C-precursors to AsA in celery tissues

From:Synthesis ofL-ascorbic acid in the phloem

Substrate
Tissue
d-[U-14C]glucose d-[U-14C]mannose l-[1-14C]galactose
Parenchyma Vascular Parenchyma Vascular Parenchyma Vascular
% Incorporation to AsAa 1.4 ± 0.06 3.1 ± 0.23 2.7 ± 0.06 5.9 ± 0.23 19.9 ± 0.92 49.8 ± 0.46
Uptake (μCi gFW-1)a 0.65 ± 0.07 3.61 ± 0.05 0.32 ± 0.02 3.49 ± 0.05 0.09 ± 0.01 0.67 ± 0.02
Uptake (nmol gFW-1)a 2.1 ± 0.23 11.6 ± 0.17 1.0 ± 0.06 11.4 ± 0.17 1.6 ± 0.12 12.2 ± 0.23
  1. aValues are presented as mean ± S.E., n = 3. Samples were prepared and incubated as described in the text. After incubation samples were extracted with 5% (w/v) PCA containing 5 mM TCEP and AsA purified as described in materials and methods. Radiolabel distribution to AsA was quantified by HPLC using online flow scintillation detection.