| Tissue |
Cycle |
AsAtin tissue (mg/100 gFW) |
AsAtin exudate (peak area mAUt) |
| Leaf |
Light |
42.5 ± 3.1 |
1.1 ± 0.1 |
|
Dark |
19.1 ± 3.0 |
0.6±0.05 |
| Tuberising stolon |
Light |
4.1 ± 0.3 |
7.9 ± 0.8 |
|
Dark |
4.5 ± 0.4 |
1.7 ± 0.9 |
- Glasshouse grown plants were transferred to off-phased controlled environment chambers 14 days prior to the start of the experiment. Environment chambers were on a 10 h dark – 14 h light cycle (see Fig. 4). At 12:00 h source leaves and tuberising stolons were removed from 3 plants and a sub-sample used for tissue AsAtquantification. For the determination of AsAtin leaf phloem exudates, petioles were re-cut under water and placed into EDTA or CaCl2exudation buffer for 90 min in a prehumidified chamber in the dark. For determination of stolon phloem exudates the cut end of the stolon attached to the plant was re-cut under water and placed in the appropriate exudation solution. Values are represented as mean ± SE, n = 6. mAUt = milli absorbance units (λ245 nm) × time.
