Table 1 Specific activity of recombinant F6H proteins^a

Step	Specific Activity (pkat/mg)
rF6Hb
Cell Lysate	0
Ni-NTA	0.091
Ni-NTA (30°C)	0.087
Ni-NTA + Superose 12	0.103
Superose 12	0.075
Cleavage of (His)₆-tag after Ni-NTA	0.044
rF6Hy (intracellular)
Cell Lysate	0
Ni-NTA	0.17
Ni-NTA + Superdex 75	0.21
Superdex 75	0.087
rF6Hys (secreted)
(NH₄)₂SO₄(35–70%)	0
(NH₄)₂SO₄+ Ni-NTA	0.11
(NH₄)₂SO₄+ Ni-NTA + Superdex 75	0.08
Superdex 75	0.04

^aAll assays were carried in the presence of assay buffer, 5 μM 3,7,4'-trimethylquercetin and ODD cofactors using the F6H enzyme assay as described in [3].