Figure 4
From:Incorporation of mammalian actin into microfilaments in plant cell nucleus
Ratio imaging of microinjected actin-Alexa Fluor®488 label and rhodamine-phalloidin staining.Microinjected actin-Alexa Fluor®488 label and phalloidin staining in an onion bulb scale epidermis cell 1 h after injection of actin-Alexa Fluor®488 conjugate, single optical slice through the central region of nuclei after deconvolution of the images,A, Alexa Fluor®488 label.B, Rhodamine-phalloidin staining.C, overlay ofA(green) andB(red).D, Cytoplasm.E, Nucleus.FandG, ratio images of green/red signals fromDandE, respectively. Black arrows, the injection site. Examples of points in the cell with high green/red ratio, green arrows; low green/red ratio, red arrows; medium green/red ratio, yellow arrows. Bars, 10 μm, color bar forFandG, relative value of green/red signals.