Figure 6

RT-PCR analysis ofAsCBFgene expression in three-week old oat. Plants were incubated at +4°C for the times indicated below the lanes (m, minutes; h, hours). Total RNA was isolated from leaves and PCR reactions were run in 30 cycles usingAsCBF1,AsCBF3, orAsCBF4特定的引物,籼稻ted to the left of the picture. Equal loading and RNA quality was controlled by amplification of the oatAsActin1gene. A 1 kb ladder was used as a size marker (not shown).