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Table 1 Transcript accumulation and activity of cell wall invertase, and sugar content in stem bases of cuttings of wild type, pHell andPhAOC-RNAi plants

From:Jasmonates act positively in adventitious root formation in petunia cuttings

parameter unit WT pHell PhAOC-RNAi
line 4 line 10 line 4 line 10 line 15
3CL9414-transcripta 2−∆Ct 20.9 ± 10.1 19.4 ± 7.5 23.1 ± 3.0 19.2 ± 4.6 14.6 ± 7.2 17.8 ± 5.4
invertase activityb pkat/mg protein 12.6 ± 3.7 14.1 ± 3.3 10.2 ± 5.2 10.1 ± 4.2 9.7 ± 3.9 8.3 ± 4.4
glucosec μmol/g f.w. 0.2 ± 0.1 0.2 ± 0.0 0.2 ± 0.1 0.2 ± 0.1 0.2 ± 0.1 0.1 ± 0.1
fructosec μmol/g f.w. 0.4 ± 0.3 0.2 ± 0.1 0.1 ± 0.0 0.5 ± 0.4 0.4 ± 0.4 0.5 ± 0.3
sucrosec μmol/g f.w. 1.8 ± 0.6 1.6 ± 0.2 1.1 ± 0.6 2.7 ± 0.9 2.3 ± 0.7 2.4 ± 1.2
  1. All data are given as mean values ± SD from eight independent, biological replicates. Data were compared by one-way-ANOVA and did not show significantly different values according to Tukey’s HSD test
  2. aTranscript data are relative toPhRSP13determined at 4 hpe
  3. bCell wall invertase activity determined at 6 hpe
  4. cSugar levels determined at 192 hpe