Fig. 4
In situhybridization ofRhAGmRNA accumulation in rose flower buds.RhAGwas detected using a DIG-labeled probe in flower buds of rose plants grown under normal temperature (25/15 °C) (a-f) or low temperature (15/5 °C) (g-l) conditions.aandg, stage 1 floral buds;bandh, stage 2 floral buds;candi, stage 3 floral buds;dandj, stage 4 floral buds. A sense probe was used for stage 3 (eandk) and stage 4 (fandl) buds as a negative control. SE, sepal; PE, petal; ST, stamen; CA, carpel. Arrows show the boundary between petal and stamen. Scale bars = 100 μm (a,b,gandh) or 200 μm (c-f;i-l)