Fig. 8

通用电气netic transformations ofArabidopsiswithGhCYP-3and identification of transgenic lines.a, Schematic representation of pBI121-GhCYP-3 vector.b, Kanamycin resistance screening of positive seedlings;c, PCR analysis of genomic DNA from Kanamycin resistant lines.d, Semi-qPCR confirmed expression ofGhCYP-3in transgenic lines.AtACT2(AT3G18780) was used as the internal standard.e, PPIase activity in wild-type (WT) and two transgenic lines. Data represent mean ± SE (n> 20) from three independent experiments. Asterisks indicate significant differences between WT and transgenic lines (Sidak's multiple comparisons test; ns= no significant, *P<0.05, **P<0.01). M, marker; L1 and L2, transgenicArabidopsislines; P, plasmid of pBI121-GhCYP-3 as positive control