Fig. 4

DEGs regulated by MC during root development in cotton.aThe total number of up- and down-regulated DEGs in root middle region and root tip at three time points.bVenn diagram displaying DEGs and common genes upon soaking-seed treatment at three time points in root middle region and root tip.cGenes differentially expressed at all three time points in root middle region and root tip. Cotton seeds of K638 were treated with deionised water (Control) or 400 mg/L MC soaking-seed for 12 h. The roots sampled at 12, 48, and 72 h after treatment were subjected to RNA-seq analyses. “R” indicates the whole root, “M” indicates the root middle region (4 to 20–40 mm from the root tip), and “T” indicates the root tip region (0 to 4 mm from the root tip). The DEGs were controlled by FDR < 5% and |log₂Fc |≥1。框中的值是日志₂Fc. 3 biological replicates per treatment, 30 roots per replicate