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Table 2 Discrimination ability of standard and species-specific barcodes and their combinations

From:Complete plastome sequencing resolves taxonomic relationships among species ofCalligonumL. (Polygonaceae) in China

DNA barcodes type length (bp) Polymorphic sites Polymorphic sites (%) Identified species (Success rate)
Standard DNA barcodes nrITS ITS1 + 5.8S 449 6 1.34 0 (0%)
5.8S + ITS2 467 16 3.43 1 (11%)
nrITS 768 22 2.92 1 (11%)
plastid matK 1527 14 0.92 2 (22%)
rbcL 1428 8 0.56 2 (22%)
trnH-psbA 386 23 5.96 2 (22%)
combination matK + rbcL 2955 22 0.74 3 (33%)
trnH-psbA + matK + rbcL 3341 44 1.32 3 (33%)
matK + rbcL +nrITS 3723 44 1.18 5 (56%)
trnH-psbA + matK + rbcL + nrITS 4109 66 1.61 4 (44%)
Genomic element trnE-T 995 21 2.11 1 (11%)
trnT-L 1026 36 3.51 2 (22%)
potential specific-barcodes ndhF 2244 38 1.69 5 (56%)
ndhF-rpl32 993 28 2.82 4 (44%)
rpl32-trnL 632 16 2.53 4 (44%)
trnC-petN 900 82 9.11 4 (44%)
trnS-G 1233 26 2.11 5 (56%)
Combination* 5795 200 3.45 6 (67%)
Complete plastid genomes (only one IR) 133,980 1151 0.86 7 (78%)