Fig. 5

ROS scavenging can reverse the hypersensitivity to salt stress of10amutants. (a) Phenotypes of wild type and10amutants in response to MV treatment. Seeds were sown on MS medium with or without the addition of 0.1 μM MV and photographed after 10 days (a) (Bar = 500 μm). Rates of cotyledon greening were calculated in (b) (means ± SD,n = 100). Asterisks indicate statistically significant differences with the corresponding wild type (**P < 0.01,t-test). (c) Alleviation of salt stress symptoms by GSH treatment in10amutants. Seedlings grown on the indicated media for ten days were photographed and the rates of cotyledon greening were calculated (d). (Bar = 500 μm. means ± SD,n = 100). Asterisks indicate statistically significant differences with the corresponding wild type (**P < 0.01,t-test). (eandf) NBT staining (e) and H₂DCF-DA staining (f) of the cotyledons of wild type and10amutants after NaCl treatment or supplemented with 100 μM GSH. Bars = 2 mm (e), bars = 300 μm (f)