Table 1 Summary of Illumina sequencing and assemblies for twoJ. vulgaris和两个J. aquaticasets

Sets^a	Total paired-end clean reads	Total assembled trinity transcripts	Transcript length range (nt^b)	GC content (%)	Contig N50^c (nt)	平均叠连群长度(nt)	Reads mapped^d(%)
Jv1*	19,725,242	152,286	301–13,238	39.37	1253	936	84.69
Jv2	36,359,675	142,213	301–13,269	39.31	1530	1132	83.25
Ja1*	20,306,518	118,936	301–15,708	39.27	1461	1082	91.57
Ja2	27,505,944	130,365	301–13,309	41.23	1441	1062	87.41

^aJv1 consisted of the pooled shoots and roots of 59 individuals ofJ. vulgaris. Jv2 was composed of shoots from five tissue culture derived plants ofJ. vulgaristreated with MeJA and five mock treated individuals. Ja1 and Ja2 were derived from the same seven individuals ofJ. aquatica, of which roots were included in Ja1 but not in Ja2
^bnt: nucleotide
^cContig N50: length such that sequence contigs of this length or longer include half the bases of the Trinity assembly
^dReads mapped: the percentage of properly paired reads mapped back to the Trinity transcriptome assembly by Bowtie2
* cDNA library was normalized before sequencing