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Fig. 9 | BMC Plant Biology

Fig. 9

From:SMRT和RNA seque Illumina公司ncing reveal novel insights into the heat stress response and crosstalk with leaf senescence in tall fescue

Fig. 9

Quantitative real time PCR (qRT-PCR) validation and function analysis of differentially expressed transcription factors.aRelative expression ofNAM-B2(i1_LQ_TF3rd_c8869/f1p0/1379),NAC029(i1_HQ_TF3rd_c26766/f2p2/1431),NAM-B1(i1_HQ_TF3rd_c44692/f2p0/1636),HsfA2d-1(i1_LQ_TF3rd_c31016/f1p0/1406),HsfA2d-2(i1_LQ_TF3rd_c80591/f1p0/1429), andHsfA2a(i3_HQ_TF3rd_c12860/f2p0/3466).bMembrane damages in agro-infiltratedNicotiana. benthamianaplants withNAC029(i1_HQ_TF3rd_c26766/f2p2/1431),NAM-B1(i1_HQ_TF3rd_c44692/f2p0/1636), andHsfA2a(i3_HQ_TF3rd_c12860/f2p0/3466) after 1 day at 45 °C by electrolyte leakage assay compared with agro-infiltrated plants with empty vector. Asterisks indicated significant difference with wild type at high temperature as estimated using Student’s t-test (*P < 0.05, ** P < 0.01). Error bars represent the standard error of the mean (n = 3)

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