Fig. 3

MutantSlAGO1Atransgenic transcripts were resistant toSlmiR168-mediated cleavage in tomatoes.a. Representation of the constructs used for transgenic expression of theSlmiR168-resistant mutant (rSlAGO1A）in tomatoes. Mutations were introduced at four locations (in red), and these base changes did not affect the native protein sequence.b. Amplification of therSlAGO1cDNA band located at 3100 bp.c. Amplification of thepri-SlmiR168aband at 159 bp