Fig. 4

Comparison of morphological changes in WT,35S:SlmiR168a‘Line 4’, and35S:rSlAGO1‘Line 2’ plants under normal K⁺conditions and K⁺deficiency stress after 7 days of treatment.a. Changes in the root hair region in WT,35S:SlmiR168a, and35S:rSlAGO1plants under CK and LK conditions after 7 days (100 × magnification) with three biological replicates.b. Differences in the fifth leaf from the bottom up in WT,35S:SlmiR168a, and35S:rSlAGO1plants under CK and LK conditions after 15 days.c. Root-shoot ratios in WT,35S:SlmiR168a, and35S:rSlAGO1plants under CK and LK conditions after 7 days with three biological replicates. * Significant differences with P < 0.05 determined using a Duncan’s test compared with the CK.d. Chlorophyll contents of the leaves in WT,35S:SlmiR168a, and35S:rSlAGO1plants under CK and LK conditions after 7 days with three biological replicates.* Significant differences with P < 0.05 determined using a Duncan’s test compared with the CK.e. K⁺contents of the roots in WT,35S:SlmiR168a, and35S:rSlAGO1plants under CK and LK conditions after 3 and 7 days with three biological replicates. * Significant differences with P < 0.05 determined using a Duncan’s test compared with the CK. CK: normal K⁺(4 mM); LK: K⁺deficiency (0.5 mM)