Fig. 4

Heterologous expression ofHpDGAT2DinChlamydomonas reinhardtiicells.aConstruct of the expression vector. PsaD-P, promoter ofPsaDgene; His tag, 6-His encoding gene; PsaD-T, terminator ofPsaDgene; RBCS2-P, promoter ofRBCS2gene; Ble, the博来霉素-resistant gene; RBCS-T, terminator ofRBCSgene.bGenomic level detection ofHpDGAT2DinC. reinhardtiicells.cTime course of biomass content under control and 1/4 N conditions.dThe transcriptional expression levels ofHpDGAT2DinC. reinhardtiicells. The gene expression levels were normalized to the endogenousactingene.eWestern blotting of HpDGAT2D-His tag fusion protein with His-tagged antibodies. Soluble and membrane proteins were separated and used for blotting. Actin which was known soluble protein was used as controls.fTAG contents and relative abundance of fatty acid inC. reinhardtiicells transformed withHpDGAT2D