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表1小RNA测序结果汇总

来自:小RNA测序提供了候选mirna -靶标对,以揭示无融合的机制花椒

样本 S1 S2 S3 S4
总读 19572690例(100%) 14258144例(100%) 13540799例(100%) 15438497例(100%)
n % > 10% 491例(0.00%) 223例(0.00%) 22 (0.00%) 41 (0.00%)
低质量 8922例(0.05%) 5375例(0.04%) 3203例(0.02%) 4338例(0.03%)
5 '适配器污染 24207例(0.12%) 18667例(0.13%) 13689例(0.10%) 14148例(0.09%)
3 '适配器为空或插入为空 943999例(4.82%) 740543例(5.19%) 263635例(1.95%) 320439例(2.08%)
采用A/T/G/C 41579例(0.21%) 10186例(0.07%) 38713例(0.29%) 10100例(0.07%)
干净的读取 18553492例(94.79%) 13483150例(94.56%) 13221537例(97.64%) 15089431例(97.74%)
总sRNA 10436878例(100%) 8007808例(100%) 10348535例(100%) 11058825例(100%)
映射sRNA 8109791例(77.70%) 7269789例(90.78%) 6456441例(62.39%) 10301776例(93.15%)
映射的sRNA 7381437例(70.72%) 6504225例(81.22%) 5689563例(54.98%) 9825133例(88.84%)
-“映射的sRNA。 728354例(6.98%) 765564例(9.56%) 766878例(7.41%) 476643例(4.31%)
  1. (1)样品:样品id。(2)总读取数:原始序列数据数。(3) N% > 10%: N含量超过10%的读次数及占总原始读次数的比例。(4)低质量:低质量阅读的数量。(5) 5 '适配器污染:5 '适配器中的读取数。(6) 3 ' adapter null or insert null:没有3 ' adapter或insert的读次数。(7)带策略A/T/G/C:包含策略A/T/G/C的读取数。(8) Clean reads:最终获得的Clean reads个数。(9)总sRNA:在每个样本中获得的读取总数。(10)映射sRNA:映射到参考序列的读数。 (11) “+” Mapped sRNA: The number of reads mapped to the same strand in the reference sequence direction. (12) “-” Mapped sRNA: The number of reads mapped to the opposite strand of the reference sequence