Fig. 7

Silencing ofBrFLUin Y-05 is critical for ALA biosynthesis.aThe phenotype of pTY plants (CK) and pTY-FLU (BrFLU-silenced) plants. Bar = 5 cm.bThe relative expression level ofBrFLUsignificantly decreased in pTY-FLU plants. The inner leaves were selected to confirm theBrFLUexpression.cThe pTY-FLU plants showed higher ALA content than pTY plants.dThe pTY-FLU plants showed higher chlorophyll content than pTY plants.eThe phenotype of pTY and pTY-FLU plants after cold acclimation. Bar = 1 cm or 2 mm.fThe pTY-FLU plants showed higher chlorophyll content than pTY plants after cold acclimation. Chlorophyll a, Chlorophyll b and total chlorophyll abbreviated as Chl a, Chl b, Total Chl, respectively. Three individual plants of each cultivar were quantified, and the chlorophyll content was measured three times. Error bars represent SE (±SE, n = 3). * representsp < 0.05, ** representsp < 0.01