Fig. 1

AtGLK1is a sucrose-inducible gene in Arabidopsis.aAccumulation ofAtGLK1mRNA under treatment with exogenous sucrose and mannitol. Seeds of the wild-type Arabidopsis (Col) were germinated and grown on 1/2 MS medium without sucrose for 4 days. The 4-day-old seedlings were then transferred to 1/2 MS medium without sucrose (Control), with 58 mM mannitol (Man), or with 2% sucrose (+ S), and each was harvested after 24 h of treatment. The total RNA was extracted and used for real-time PCR.bEvaluation of the GUS expression inAtGLK1::GUStransgenic Arabidopsis seedlings treated with exogenous sucrose and mannitol.AtGLK1::GUStransgenic Arabidopsis seeds from two representative lines (AtGLK1::GUS-1 and -2) were germinated and grown on 1/2 MS medium lacking sucrose. On the 4th day after germination, The seedlings were transferred to 1/2 MS medium without sucrose (Control), with 58 mM mannitol (Man), or with 2% sucrose (+ S), and grown for an additional 24 h, and then incubated in a GUS-staining solution. The asterisk indicates statistically significant differences compared with the control (Student’sttest: *P < 0.05)