Fig. 8
The spot assays for stress tolerance confirmation of theCrPIP1;5expression in yeast.aMap of the CrPIP1;5-pYES-DEST52 construct.bHigh osmotic stress and salt tolerance confirmation in yeast. The growth state of yeast cells (WT, BY4741) transformed with CrPIP1;5-pYES-DEST52 and empty vector pYES2 with or without PEG8000, sorbitol, or NaCl on SDG-Ura plates. The concentration of these stress factors for each treatment was labeled on the left.cH2O2oxidative stress tolerance confirmation in yeast. The growth state of yeast cells (WT and H2O2-sensitive mutant strainskn7Δ) transformed with CrPIP1;5-pYES-DEST52 and empty vector pYES2 with or without H2O2on SDG-Ura plates. The concentration of H2O2for each treatment was labeled on the left