Fig. 10

Calcofluor white staining of microspores from wild-type (WT) andFLA14-overexpressing (OE) transgenic Arabidopsis plants.a-d, f-iMicrospores from WT plants.f-iThe corresponding bright field images.eAn anther at anthesis stage from OE plants. Asterisks indicate aberrant pollen grains with a significant increase in the calcofluor white fluorescent signals.j-sMicrospores from OE plants.o-sThe corresponding bright field images.l, m, q, rArrows indicate the aborted pollen grains with no detectable calcofluor white fluorescence.n, sComparison of aberrant pollen grains with unusually strong calcofluor white fluorescence (indicated by asterisks) and normal mature pollen grains from OE plants.a, f, j, oEarly uninucleate stage.b, g, k, pLate uninucleate stage.c, h, l, qBicellular stage.d, i, m, rTricellular stage.e, n, sAnthesis stage. Scale bars = 20 μm (a-d, f-s); 50 μm (e)